Improved resolution of bacteria by high throughput sequence analysis of the rRNA internal transcribed spacer
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence analysis of the Second Internal Transcribed Spacer (ITS2) in Hyalomma anatolicum anatolicum in Iran
Ticks are important acarina that infest animals. They are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. Moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. In view of the importance of Hyalomma anatolicum anatolicum in pathogen transmission, including Th...
متن کاملHigh-resolution differentiation of Cyanobacteria by using rRNA-internal transcribed spacer denaturing gradient gel electrophoresis.
For many ecological studies of cyanobacteria, it is essential that closely related species or strains can be discriminated. Since this is often not possible by using morphological features, cyanobacteria are frequently studied by using DNA-based methods. A powerful method for analysis of the diversity and dynamics of microbial populations and for checking the purity and affiliation of cultivate...
متن کاملnucleotide sequence analysis of the second internal transcribed spacer (its2) in hyalomma anatolicum anatolicum in iran
ticks are important acarina that infest animals. they are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. in view of the importance of hyalomma anatolicum anatolicum in pathogen transmission, including th...
متن کاملMolecular Identification of Two Strains of Phellinus sp. by Internal Transcribed Spacer Sequence Analysis
Two species of cultivated Phellinus sp. were identified as P. baumii by internal transcribed spacer (ITS) sequence analysis. The fruit bodies of the examined strains were similar to those of naturally occurring strains, having a bracket-like form, yellow-to-orange color, and poroid hymenial surfaces. The DNA sequences of ITS region of both strains showed a homology of 99% with ITS1 to ITS2 sequ...
متن کاملSequence Analysis of the Internal Transcribed Spacer of Ribosomal DNA in the Genus Rhizopus
The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with 154~155 bp. The sequence of ITS2 was mo...
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ژورنال
عنوان ژورنال: Journal of Microbiological Methods
سال: 2014
ISSN: 0167-7012
DOI: 10.1016/j.mimet.2014.07.001